Identification of Chemo and Radio-Resistant Sub-Population of Stem Cells in Human Cervical Cancer HeLa Cells
Nabil Mohie Abdel‐Hamid, Moustafa Fathy, Chika Koike, Toshiko Yoshida, Motonori Okabe, Kaixuan Zho, Mekky M. Abouzied, Toshio Nikaido
Abstract
BACKGROUND: Cervical cancer ranks the second female malignancy after breast cancer. Cancer stem cells (CSCs) are hard to be eradicated, so can recur. We aim to isolate and characterize CSCs from HeLa cells. METHODS: These cells express clusters of differentiation (CDs), 44 and 24, to be sorted by fluorescence-activated cell sorting (FACS). RESULTS: cells showed potential to form spheres, tumorigenicity, stemness genes and higher resistance to cisplatin, X-ray. CONCLUSION: studies, suggesting that targeting may lead to screening of new anti-cancer therapies.
Topics & Concepts
HeLaCD44Cancer stem cellCancer researchCD24CancerStem cellPopulationCell sortingCancer cellAbcg2CisplatinMalignancyIn vitroBiologySide populationChemistryMedicinePathologyCell biologyGeneGeneticsChemotherapyTransporterATP-binding cassette transporterEnvironmental healthCancer Cells and MetastasisMicroRNA in disease regulationCancer Genomics and Diagnostics