Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RubisCO) Is Essential for Growth of the Methanotroph Methylococcus capsulatus Strain Bath
Calvin A. Henard, Chao Wu, Wei Xiong, Jessica M. Henard, Brett Davidheiser‐Kroll, Fabini D. Orata, Michael T. Guarnieri
Abstract
The importance of RubisCO and CO 2 in M. capsulatus Bath metabolism is unclear. In this study, we demonstrated that both CO 2 and RubisCO are essential for M. capsulatus Bath growth. 13 CO 2 tracing experiments supported that RubisCO mediates CO 2 fixation and that a noncanonical Calvin cycle is active in this organism.
Topics & Concepts
Ribulose 1,5-bisphosphateOxygenaseRuBisCOPyruvate carboxylaseBiochemistryBiologyMethanotrophChemistryStrain (injury)EnzymeAnatomyAnaerobic oxidation of methaneCatalysisMicrobial metabolism and enzyme functionMicrobial bioremediation and biosurfactantsPorphyrin Metabolism and Disorders