Comparative appraisal of HPLC, Chloramine‐T and Lane–Eynon methods for quantification of carbohydrates in concentrated dairy products
Luana Cristina Andrade da Silva, José A Q Lafetá, Maurício de Oliveira Leite, Edimar Aparecida Filomeno Fontes, Jane Sélia dos Reis Coimbra
Abstract
Lactose and sucrose were quantified in sweetened condensed milk and ‘Dulce de leche’ using a high‐performance liquid chromatography (HPLC) approach as compared with the classical methods (Chloramine‐T and Lane–Eynon). Lane–Eynon method estimated the lowest values of carbohydrates for both products. The chromatographic method quantified the carbohydrate contents at the same range as Chloramine‐T method. The values of the estimated standard error (ESE) for HPLC versus Chloramine‐T (0.55–0.77 mass %) were lower than the ESE for HPLC versus Lane–Eynon (1.01–1.63 mass %). Calcium concentration in the products may explain underestimated carbohydrate contents by the Lane–Eynon method.