Minichromosome maintenance protein 5 is an important pathogenic factor of oral squamous cell carcinoma
Miao Hao, Huiyu Wang, Chu Zhang, Chunyan Li, Xiaofeng Wang
Abstract
Oral squamous cell carcinoma is one of the most common causes of malignancy‑associated death. Early diagnosis of oral squamous cell carcinoma (OSCC) is important in patient treatment and prognostic evaluation. Due to the lack of significant therapeutic benefit, the 5‑year survival rate has not improved. Therefore, effective novel markers are needed to improve diagnosis. To determine novel promising diagnostic biomarkers for OSCC, 416 upregulated and 416 downregulated differentially expressed genes were screened from OSCC tissues using an RNA microarray. The results suggested that minichromosome maintenance protein (<em>MCM5</em>) mRNA was significantly overexpressed in OSCC tissues compared with that in adjacent normal tissues. Moreover, silencing of MCM5 expression an OSCC cell line (SCC‑15) significantly impaired proliferation and colony formation. Furthermore, negative regulation of the mRNA and protein expression of MCM5 and demonstrated that <em>MCM5</em> served as a cancer‑promoting gene modulating OSCC cell proliferation through induced G<sub>2</sub>/M phase arrest. In this process, the mRNA expression of cyclin E and cyclin‑dependent kinase 2 was downregulated, while p21 expression was upregulated. These results suggested that MCM5 may be an important pathogenic factor of OSCC. High expression levels of MCM5 may serve as a marker for the early diagnosis of OSCC.