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Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome editing

Roman Teo Oliynyk, George M. Church

2025Nature Protocols11 citationsDOI

Topics & Concepts

CRISPRGenome editingCas9PlasmidSynthetic biologyComputational biologyGuide RNABiologyDNARNACircular RNAGeneticsGeneCRISPR and Genetic EngineeringInnovation and Socioeconomic DevelopmentPluripotent Stem Cells Research
Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome editing | Litcius