<scp>MPK12</scp> in stomatal <scp>CO<sub>2</sub></scp> signaling: function beyond its kinase activity
Chung‐Yueh Yeh, Yuh‐Shuh Wang, Yohei Takahashi, Katarina Kuusk, Karnelia Paul, Triinu Arjus, Oleksii Yadlos, Julian I. Schroeder, Ivar Ilves, Alfonso T. García‐Sosa, Hannes Kollist
Abstract
Summary Protein phosphorylation is a major molecular switch involved in the regulation of stomatal opening and closure. Previous research defined interaction between MAP kinase 12 and Raf‐like kinase HT1 as a required step for stomatal movements caused by changes in CO 2 concentration. However, whether MPK12 kinase activity is required for regulation of CO 2 ‐induced stomatal responses warrants in‐depth investigation. We apply genetic, biochemical, and structural modeling approaches to examining the noncatalytic role of MPK12 in guard cell CO 2 signaling that relies on allosteric inhibition of HT1. We show that CO 2 /HCO 3 − ‐enhanced MPK12 interaction with HT1 is independent of its kinase activity. By analyzing gas exchange of plant lines expressing various kinase‐dead and constitutively active versions of MPK12 in a plant line where MPK12 is deleted, we confirmed that CO 2 ‐dependent stomatal responses rely on MPK12's ability to bind to HT1, but not its kinase activity. We also demonstrate that purified MPK12 and HT1 proteins form a heterodimer in the presence of CO 2 /HCO 3 − and present structural modeling that explains the MPK12:HT1 interaction interface. These data add to the model that MPK12 kinase‐activity‐independent interaction with HT1 functions as a molecular switch by which guard cells sense changes in atmospheric CO 2 concentration.