Effect of different stainless steel surfaces on the formation and control of Vibrio parahaemolyticus biofilm
Sumate Tantratian, Nattanit Srimangkornkaew, Chuenjit Prakitchaiwattana, Romanee Sanguandeekul
Abstract
The attachment, biofilm formation and control of biofilm formed by Vibrio parahaemolyticus (VP) ATCC 17802 on four stainless steel (SS) coupons which are different in type or finishing, 316L, 304, 430 with 2B finish and 304 with BA finish, were studied. SS coupons were subjected to surface roughness measurement using Atomic Force Microscope and Scanning Electron Microscope. The surface of 316L/2B was the roughest surface while 403/2B and 304/BA were the least roughness surface. VP were able to attach on all SS surfaces immediately after contact. They tended to attach on the rougher surface of the SS samples better than the smoother ones. They could be released from surfaces into liquid medium and grew very fast, after contact. Attached cells formed multilayer clumps within 72 h. Cells in biofilms survived on all SS surfaces under dry condition for more than 72 h. The 0.02% benzalkonium chloride (BAC) completely eliminate 8 log10 CFU mL−1 planktonic cells immediately after contact, but it took more than 1 min to eliminate viable cells of 24 and 72h biofilms from 304/2B and 316L/2B. The viable cells in biofilm on the rougher surfaces showed more resistance to the BAC than those on the smoother ones.