Amygdalobacter indicium gen. nov., sp. nov., and Amygdalobacter nucleatus sp. nov., gen. nov.: novel bacteria from the family Oscillospiraceae isolated from the female genital tract
Sujatha Srinivasan, Michele N. Austin, Tina L. Fiedler, Susan Strenk, Kathy Agnew, G. A. Nagana Gowda, Daniel Raftery, May A. Beamer, Sharon L. Achilles, Harold C. Wiesenfeld, David N. Fredricks, Sharon L. Hillier
Abstract
Four obligately anaerobic Gram-positive bacteria representing one novel genus and two novel species were isolated from the female genital tract. Both novel species, designated UPII 610-J T and KA00274 T , and an additional isolate of each species were characterized utilizing biochemical, genotypic and phylogenetic analyses. All strains were non-motile and non-spore forming, asaccharolytic, non-cellulolytic and indole-negative coccobacilli. Fatty acid methyl ester analysis for UPII 610-J T and KA00274 T and additional isolates revealed C 16 : 0 , C 18 : 0 , C 18:1 ω9c and C 18:2 ω6,9c to be the major fatty acids for both species. UPII 610-J T had a 16S rRNA gene sequence similarity of 99.4 % to an uncultured clone sequence (AY724740) designated as Bacterial Vaginosis Associated Bacterium 2 (BVAB2). KA00274 T had a 16S rRNA gene sequence similarity of 96.5 % to UPII 610-J T . Whole genomic DNA mol% G+C content was 42.2 and 39.3 % for UPII 610-JT and KA00274 T , respectively. Phylogenetic analyses indicate these isolates represent a novel genus and two novel species within the Oscillospiraceae family. We propose the names Amygdalobacter indicium gen. nov., sp. nov., for UPII 610-J T representing the type strain of this species (=DSM 112989 T , =ATCC TSD-274 T ) and Amygdalobacter nucleatus gen. nov., sp. nov., for KA00274 T representing the type strain of this species (=DSM 112988 T , =ATCC TSD-275 T ).