Dual Channel Microfluidics for Mimicking the Blood–Brain Barrier
Boris Buchroithner, Sandra Mayr, Fabian Hauser, Eleni Priglinger, Herbert Stangl, Ana Raquel Santa-Maria, Mária A. Deli, András Dér, Thomas A. Klar, Markus Axmann, Dmitry Sivun, Mario Mairhofer, Jaroslaw Jacak
Abstract
laser cutting with subsequent structuring using 3D multiphoton lithography for improved cell growth. The upper channel was loaded with endothelial cells under flow conditions, which showed polarization and junction formation. A coculture of human vascular endothelial cells with pericytes was developed that mimics the blood-brain barrier. Finally, this dual channel microfluidics system enabled 3D localization microscopy of the cytoskeleton and 3D single-molecule-sensitive tracing of lipoprotein particles.
Topics & Concepts
MicrofluidicsOptical tweezersMaterials scienceCytoskeletonNanotechnologyMicroscopyNumerical apertureMembraneBiophysicsChemistryCellOptoelectronicsOpticsBiologyPhysicsBiochemistryWavelengthAdvanced Fluorescence Microscopy TechniquesCell Image Analysis TechniquesCellular Mechanics and Interactions