A beginner’s guide to mass spectrometry–based proteomics
Ankit Sinha, Matthias Mann
Abstract
Mass spectrometry (MS)-based proteomics is the most comprehensive approach for the quantitative profiling of proteins, their interactions and modifications. It is a challenging topic as a firm grasp requires expertise in biochemistry for sample preparation, analytical chemistry for instrumentation and computational biology for data analysis. In this short guide, we highlight the various components of a mass spectrometer, the sample preparation process for conversion of proteins into peptides, and quantification and analysis strategies. The advancing technology of MS-based proteomics now opens up opportunities in clinical applications and single-cell analysis.
Topics & Concepts
ProteomicsMass spectrometryGRASPQuantitative proteomicsProfiling (computer programming)ChemistryComputer scienceInstrumentation (computer programming)Sample preparationData scienceComputational biologyChromatographyBiologyBiochemistrySoftware engineeringOperating systemGeneAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies