Semisynthetic maturation of [FeFe]-hydrogenase using [Fe<sub>2</sub>(μ-SH)<sub>2</sub>(CN)<sub>2</sub>(CO)<sub>4</sub>]<sup>2−</sup>: key roles for HydF and GTP
Batuhan Balci, Roark D. O’Neill, Eric M. Shepard, Adrien Pagnier, Alexander Marlott, Michael T. Mock, William E. Broderick, Joan Broderick
Abstract
together with HydF and components of the glycine cleavage system, but in the absence of the maturases HydE and HydG. This semisynthetic and fully-defined maturation provides new insights into the nature of H-cluster biosynthesis.
Topics & Concepts
HydrogenaseCleavage (geology)ChemistryCrystallographyStereochemistryCatalysisMaterials scienceBiochemistryComposite materialFracture (geology)Metalloenzymes and iron-sulfur proteinsElectrocatalysts for Energy ConversionHydrogen Storage and Materials