Effects of storage conditions on the performance of an electrochemical aptamer-based sensor
Julia Chung, Adriana Billante, Charlotte Flatebo, Kaylyn K. Leung, Julian Gerson, Nicole Emmons, Tod E. Kippin, Lior Sepunaru, Kevin W. Plaxco
Abstract
in the living body, and (3) independent of the chemical or enzymatic reactivity of its target, rendering it adaptable to a wide range of analytes. These attributes suggest the EAB platform will prove to be an important tool in both biomedical research and clinical practice. To advance this possibility, here we have explored the stability of EAB sensors upon storage, using retention of the target recognizing aptamer, the sensor's signal gain, and the affinity of the aptamer as our performance metrics. Doing so we find that low-temperature (-20 °C) storage is sufficient to preserve sensor functionality for at least six months without the need for exogenous preservatives.