CRISPR Cas12a-enabled biosensors coupled with commercial pregnancy test strips for the visible point-of-care testing of SARS-CoV-2
Peijie Shen, Zhenjun Si, Di Huang, Zhipeng Xu, Ziyi Wang, Mengjun Fang, Zhinan Xu
Abstract
RT-qPCR in spiked samples. With the advantages of low reagent cost, simple operation procedure, and visible signal output, CRISPR-PTS assay was expected to provide a strong supplement in the prevention and early diagnosis of infectious diseases in resource-limited situations.
Topics & Concepts
CRISPRPoint-of-care testingLoop-mediated isothermal amplificationNucleic acidSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Point of careNucleic acid detectionComputational biologyCoronavirus disease 2019 (COVID-19)VirologyChemistryBiologyInfectious disease (medical specialty)MedicineDiseaseGeneImmunologyDNAPathologyBiochemistryCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesSARS-CoV-2 detection and testing