Universal quenching of common fluorescent probes by water and alcohols
Jimmy Maillard, Kathrin Klehs, Christopher A. Rumble, Eric Vauthey, Mike Heilemann, Alexandre Fürstenberg
Abstract
Overtones and combinations of O–H vibrations in the solvent efficiently quench red-emitting fluorophores by resonant energy transfer.
Topics & Concepts
FluorescenceQuenching (fluorescence)ChemistryAqueous solutionPhotochemistryAqueous mediumLabellingOrganic chemistryBiochemistryOpticsPhysicsMolecular Sensors and Ion DetectionLanthanide and Transition Metal ComplexesAdvanced Fluorescence Microscopy Techniques