Development of a Novel<i>In Vitro</i>Primary Human Monocyte-Derived Macrophage Model To Study Reactivation of HIV-1 Transcription
Michelle E. Wong, Chad Johnson, Anna C. Hearps, Anthony Jaworowski
Abstract
Agents which modulate latent HIV reservoirs in infected cells are of considerable interest to HIV cure strategies. The present study characterizes a robust, reproducible model enabling quantification of HIV reactivation in primary HIV-infected human MDM which is relatively insensitive to the monocyte donor source and hence suitable for evaluating latency modifiers in MDM.
Topics & Concepts
BiologyInfectivityIn vitroVirologyMonocyteViremiaMolecular biologyTumor necrosis factor alphaVirus latencyCytokineCell cultureHeterologousImmunologyViral replicationHuman immunodeficiency virus (HIV)VirusGeneGeneticsBiochemistryHIV Research and TreatmentImmune Cell Function and InteractionImmune cells in cancer