Determining the Stoichiometry of Small Protein Oligomers Using Steady-State Fluorescence Anisotropy
Philipp J. Heckmeier, Ganesh Agam, Mark G. Teese, Maria Hoyer, Ralf Stehle, Don C. Lamb, Dieter Langosch
Topics & Concepts
PhotobleachingFluorescence anisotropyFörster resonance energy transferFluorescenceChemistryStoichiometryAnisotropyMonomerCovalent bondExcited stateGreen fluorescent proteinChemical physicsPhotochemistryCrystallographyMembranePolymerPhysical chemistryAtomic physicsPhysicsOpticsGeneOrganic chemistryBiochemistryPhotosynthetic Processes and MechanismsAdvanced Fluorescence Microscopy TechniquesProtein Structure and Dynamics