Diatom modulation of select bacteria through use of two unique secondary metabolites
Ahmed A. Shibl, Ashley Isaac, Michael A. Ochsenkühn, Anny Cárdenas, Cong Fei, Gregory Behringer, Marc Arnoux, Nizar Drou, Miraflor P. Santos, Kristin C. Gunsalus, Christian R. Voolstra, Shady A. Amin
Abstract
reprograms its transcriptional and metabolic profiles in response to bacteria to secrete a suite of central metabolites and two unusual secondary metabolites, rosmarinic acid and azelaic acid. While central metabolites are utilized by potential bacterial symbionts and opportunists alike, rosmarinic acid promotes attachment of beneficial bacteria to the diatom and simultaneously suppresses the attachment of opportunists. Similarly, azelaic acid enhances growth of beneficial bacteria while simultaneously inhibiting growth of opportunistic ones. We further show that the bacterial response to azelaic acid is numerically rare but globally distributed in the world's oceans and taxonomically restricted to a handful of bacterial genera. Our results demonstrate the innate ability of an important unicellular eukaryotic group to modulate select bacteria in their microbial consortia, similar to higher eukaryotes, using unique secondary metabolites that regulate bacterial growth and behavior inversely across different bacterial populations.