PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes
Hongjun Zhang, Jiao Zhou, Peng Xiao, Yong Lin, Xin Gong, Shiyan Liu, Qingjia Xu, Minjin Wang, Haiyan Ren, Mengji Lu, Yuan Wang, Jing Zhu, Zhiping Xie, Huihui Li, Kefeng Lu
Abstract
Ape1: aminopeptidase Ι; ATG: autophagy related; EBSS: Earle's balanced salt solution; ER: endoplasmic reticulum; ERGIC: Golgi apparatus and ER-Golgi intermediate compartment; HOPS: homotypic fusion and protein sorting complex; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; PtdIns3P: phosphatidylinositol-3-phosphate; PtdIns4K: phosphoinositide-4-kinase; PtdIns4P: phosphatidylinositol-4-phosphate; SD-N: nitrogen starvation medium; SNARE: soluble N-ethylamide-sensitive factor attachment protein receptor.
Topics & Concepts
BiologyCell biologyAutophagyEndoplasmic reticulumGolgi apparatusLysosomeAutophagosomeVacuolar protein sortingLipid bilayer fusionPhosphatidylinositolVacuoleBiochemistryCytoplasmPhosphorylationMembraneEnzymeApoptosisAutophagy in Disease and TherapyCellular transport and secretionEndoplasmic Reticulum Stress and Disease