Chemical synthesis of stimuli-responsive guide RNA for conditional control of CRISPR-Cas9 gene editing
Chunmei Gu, Lu Xiao, Jiachen Shang, Xiao Xu, He Luo, Yu Xiang
Abstract
-methylribonucleotide phosphorothioate (PS-2'-OMe). We demonstrated CRISPR-Cas9-mediated gene editing in human cells triggered by oxidative stress and visible light, respectively. Our study tackles the synthetic challenge and paves the way for chemically modified RNA to play more active roles in gene therapy.
Topics & Concepts
CRISPRCas9RNAGeneComputational biologyGuide RNABiologyGeneticsComputer scienceCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms