An Easy-to-Use Plasmid Toolset for Efficient Generation and Benchmarking of Synthetic Small RNAs in Bacteria
Tania S. Köbel, Rafael Melo Palhares, Christin Fromm, Witold Szymański, Georgia Angelidou, Timo Glatter, Jens Georg, Bork A. Berghoff, Daniel Schindler
Abstract
and rendering cells sensitive toward the β-lactam antibiotic oxacillin. We further highlight that it may be necessary to test multiple seed regions and sRNA scaffolds to achieve the desired regulatory effect. The described plasmid toolset allows quick construction and testing of various synthetic sRNAs based on the user's needs.
Topics & Concepts
Synthetic biologyComputational biologyPlasmidBiologyEscherichia coliComputer scienceGeneGeneticsBacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsBacteriophages and microbial interactions