Generation of an induced pluripotent stem cell line (UNCCi002-A) from a healthy donor using a non-integration system to study Cerebral Cavernous Malformation (CCM)
Alvaro A. Beltran, Alvaro A. Beltran, Sarahi G. Molina, Ariana Marquez Gonzalez, Lorena J. Munoz, Jose F. Olivares, Adriana S. Beltran, Adriana S. Beltran
Abstract
The generation of induced pluripotent stem cells (iPSCs) from healthy individuals is an invaluable resource as reference control in disease modeling and drug discovery. This paper details the reprogramming of peripheral blood mononuclear cells (PBMCs) isolated from a healthy 27 years-old male using non-integration technology. The derived iPSCs displayed typical pluripotent stem cell morphology, the capacity to differentiate into the three germ layers, and normal karyotype. This iPSC line will be used as a reference control to study the Cerebral Cavernous Malformation disease mechanism.
Topics & Concepts
Induced pluripotent stem cellReprogrammingBiologyGerm layerPeripheral blood mononuclear cellStem cellKaryotypeImmunologyCellCell biologyCancer researchEmbryonic stem cellGeneticsGeneChromosomeIn vitroVascular Malformations Diagnosis and TreatmentIntracranial Aneurysms: Treatment and ComplicationsAdvanced Memory and Neural Computing