H-NS Represses Biofilm Formation and c-di-GMP Synthesis in <i>Vibrio parahaemolyticus</i>.
Xing Fan Xue, Miao Miao Zhnag, Jun Fang Sun, Xue Li, Qi Min Wu, Zhe Yin, Wen Yang, Bin Ni, Ling Fei Hu, Zhou Dong, Ren Fei Lu, Yi Quan Zhang
Abstract
Objective: RIMD2210633. Methods: Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay. Results: , VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism. Conclusion: repression of the production of intracellular c-di-GMP.
Topics & Concepts
Vibrio parahaemolyticusBiofilmNucleoidCrystal violetOperonIntracellularChemistryTranscription (linguistics)Molecular biologyMicrobiologyBiologyBacteriaGeneBiochemistryEscherichia coliGeneticsLinguisticsPhilosophyVibrio bacteria research studiesBacterial biofilms and quorum sensingOral microbiology and periodontitis research