In situ detection of protein-protein interaction by proximity ligation assay in patient derived brain tumor stem cells
Ahmad Sharanek, Laura Raco, Vahab D. Soleimani, Arezu Jahani‐Asl
Abstract
using fluorescent microscopy. This PLA protocol is optimized specifically for BTSCs and includes key controls for effective result analysis. For complete details on the use and execution of this protocol, please refer to Sharanek et al. (2021).
Topics & Concepts
Proximity ligation assayStem cellLigationIn situProtocol (science)Computational biologyChemistryBiologyCancer researchCell biologyMolecular biologyPathologyMedicineBiochemistryOrganic chemistryAlternative medicineReceptorUbiquitin and proteasome pathwaysGalectins and Cancer BiologyRNA Interference and Gene Delivery