Baicalin ameliorates the gut barrier function and intestinal microbiota of broiler chickens
Shuangxiu Wan, Linzheng Wang, Zhili Hao, Lin Zhu, Xiaoxia Mao, Hongquan Li, Panpan Sun, Wei Yin, Kuohai Fan, Hailong Zhang, Beibei Li, Wansen Nie, Zongjie Li, Nian X. Sun
Abstract
<p indent="0mm">The deoxynivalenol (DON)-contaminated feeds can impair chicken gut barrier function, disturb the balance of the intestinal microbiota, decrease chicken growth performance and cause major economic loss. With the aim of investigating the ameliorating effects of baicalin on broiler intestinal barrier damage and gut microbiota dysbiosis induced by DON, a total of 150 Arbor Acres broilers are used in the present study. The morphological damage to the duodenum, jejunum, and ileum caused by DON is reversed by treatment with different doses of baicalin, and the expression of tight junction proteins (ZO-1, claudin-1, and occludin) is also significantly increased in the baicalin-treated groups. Moreover, the disturbance of the intestinal microbiota caused by DON-contaminated feed is altered by baicalin treatment. In particular, compared with those in the DON group, the relative abundances of <italic> Lactobacillus</italic>, <italic>Lachnoclostridium</italic>, <italic> Ruminiclostridium</italic> and other beneficial microbes in the baicalin-treated groups are significantly greater. However, the percentage of <italic> unclassified_f__Lachnospiraceae</italic> in the baicalin-treated groups is significantly decreased in the DON group. Overall, the current results demonstrate that different doses of baicalin can improve broiler intestinal barrier function and the ameliorating effects on broiler intestinal barrier damage may be related to modulations of the intestinal microbiota.