Co-production of xylooligosaccharides and glucose by multiple-joint enzymes treatment based on maleic acid pretreated sugarcane bagasse
Weiwei Lei, Yuejie Zhai, Lei Zhang, Shuangquan Yao, Caoxing Huang, Yong Xu, Xin Zhou
Abstract
In this study, the combination method of acidic pre-hydrolysis and endo-xylanase hydrolysis was proven to efficiently produce xylooligosaccharides (XOS) from sugarcane bagasse (SCB). The maximum yield of XOS from SCB was achieved 60.5 % by a combination of acidic hydrolysis (0.26 % maleic acid (MA) concentration, temperature 147.5 °C, and duration 40 min) and additional endo-xylanase hydrolysis. Meanwhile, the proportion of the predominant active constituents (xylobiose and xylotriose) significantly increased over 80.0 %. In addition, the efficient enzymatic saccharification of pretreated materials is a key step in achieving economics of lignocellulosic biorefinery, however, the remaining solids from XOS preparation cannot be directly achieved a satisficing cellulose saccharification efficiency (<80.0 %) with cellulase. Therefore, in order to enhance the saccharification efficiency, ferulic acid esterase, which could break the bonds of lignin-carbohydrate complex, was co-loaded with cellulase. As a result, the cellulose saccharification efficiency was improved to 91.5 %. Overall, favorable outcomes suggested that the multiple-joint enzymes treatment based on maleic acid pretreated SCB was a green and cost-effective method for sugar products (XOS and glucose) preparation. • Cooperation of acidic and xylanase hydrolysis can achieve higher XOS yield. • The maximum XOS yield was 59.5 % and the main components were X2 and X3. • The XOS and glucose can be efficiently produced by multiple-enzymes assistance.