Landscape of Post-Transcriptional tRNA Modifications in Streptomyces albidoflavus J1074 as Portrayed by Mass Spectrometry and Genomic Data Mining
Oksana Koshla, Lea‐Marie Vogt, Oleksandr Rydkin, Yuliia Sehin, Iryna Ostash, Mark Helm, Bohdan Ostash
Abstract
, even though peculiar tRNA-based regulatory mechanisms operate in this taxon. We provide a first detailed description of the chemical diversity of PTTMs in the model species, S. albidoflavus J1074, and identify most plausible genes for these PTTMs. Some of the PTTMs are described for the first time for Streptomyces. Production of certain PTTMs in J1074 appears to depend on enzymes that show no sequence similarity to known PTTM enzymes from model species. Our findings are of relevance for interrogation of genetic basis of PTTMs in pathogenic actinobacteria, such as M. tuberculosis.
Topics & Concepts
Transfer RNABiologyStreptomycesStreptomyces coelicolorIn silicoBacillus subtilisBiochemistryGeneticsSecondary metabolismEscherichia coliEnzymeBacteriaBiosynthesisRNAGeneRNA modifications and cancerRNA and protein synthesis mechanismsGenomics and Phylogenetic Studies