Structural Studies Reveal the Role of Helix 68 in the Elongation Step of Protein Biosynthesis
Giuseppe Cimicata, G. Fridkin, Tanaya Bose, Zohar Eyal, Yehuda Halfon, Elinor Breiner-Goldstein, Tara Fox, Ella Zimmerman, Anat Bashan, Natalia de Val, Alexander Wlodawer, Ada Yonath
Abstract
The mechanism that regulates the translocation step in ribosomes during protein synthesis is not fully understood. In this work, cryo-EM techniques used to image ribosomes from Staphylococcus aureus after incubation at physiological temperature allowed the identification of a conformation of the helix 68 that has never been observed so far.
Topics & Concepts
RibosomeEukaryotic RibosomeProtein biosynthesisRibosomal RNAPeptidyl transferaseBiologyCell biologyRNAHelix (gastropod)BiophysicsTranslation (biology)Ribosomal proteinBiochemistryProtein subunitChemistryMessenger RNAGeneEcologySnailRNA and protein synthesis mechanismsRNA modifications and cancerRNA Research and Splicing