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Sequential single-enzyme oxidation of 5-hydroxymethylfurfural to 2,5-furandicarboxylic acid by an engineered lanthanide-dependent alcohol dehydrogenase

Ke Liu, Ling Jiang, Lun Wang, Qunfeng Zhang, Lirong Yang, Jianping Wu, Haoran Yu

2025Green Chemistry12 citationsDOIOpen Access PDF

Abstract

A lanthanide-dependent alcohol dehydrogenase, PedH, was identified, which possessed the rare ability to continuously oxidize HMF to produce FDCA. A fully automated workflow was developed to modify PedH, resulting in an increased yield of FDCA.

Topics & Concepts

Alcohol dehydrogenaseLanthanideChemistryEnzymeAlcohol oxidoreductaseAlcoholDehydrogenaseAlcohol oxidation5-hydroxymethylfurfuralOrganic chemistryBiochemistryCatalysisIonNAD+ kinaseCatalysis for Biomass ConversionEnzyme Catalysis and ImmobilizationBiofuel production and bioconversion
Sequential single-enzyme oxidation of 5-hydroxymethylfurfural to 2,5-furandicarboxylic acid by an engineered lanthanide-dependent alcohol dehydrogenase | Litcius