Aptamer-Initiated Catalytic Hairpin Assembly Fluorescence Assay for Universal, Sensitive Exosome Detection
Jiaqi Zhou, Qiuyuan Lin, Zhipeng Huang, Huiwen Xiong, Bin Yang, Hui Chen, Jilie Kong
Abstract
particles/μL and a low detection limit (LOD) of 0.5 particles/μL. The universality of the AICHA method was verified for PANC-1 cell-derived exosomes, the LOD of which was determined to be 0.1 particles/μL. The performances in serum samples were detected with a recovery rate range of 95.45-106.2%, which demonstrates its significant potential for protein biomarker analysis and cancer diagnosis.
Topics & Concepts
ChemistryAptamerFluorescenceExosomeNanotechnologyBiophysicsBiochemistryMolecular biologyMicrovesiclesmicroRNAOpticsGeneBiologyMaterials sciencePhysicsExtracellular vesicles in diseaseAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery