Identification and in silico bioinformatics analysis of <scp>PR10</scp> proteins in cashew nut
Shanna Bastiaan‐Net, M.C. Pina-Pérez, Bas J. W. Dekkers, Adrie H. Westphal, Antoine H. P. America, Renata M.C. Ariëns, Nicolette W. de Jong, Harry J. Wichers, Jurriaan J. Mes
Abstract
Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis-related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography-mass spectrometry (LC-MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.