Coupling Droplet Microfluidics with Mass Spectrometry for Ultrahigh-Throughput Analysis of Complex Mixtures up to and above 30 Hz
Emily Kempa, Clive A. Smith, Xin Li, Bruno Bellina, Keith Richardson, Steven Pringle, James L. Galman, Nicholas J. Turner, Perdita E. Barran
Abstract
, on a DTIMS Q-TOF similar rates were obtained, and on a TWIMS Q-TOF utilizing IM-MS software rates up to 33 Hz are demonstrated. The potential of this approach is demonstrated with proof of concept experiments on crude mixtures including egg white, unpurified recombinant protein, and a biotransformation supernatant.
Topics & Concepts
ChemistryMicrofluidicsMass spectrometryOrbitrapThroughputCoupling (piping)ChromatographyDrug discoveryNanotechnologyAnalytical Chemistry (journal)Detection limitComputer scienceWirelessTelecommunicationsEngineeringMechanical engineeringMaterials scienceBiochemistryInnovative Microfluidic and Catalytic Techniques InnovationMicrofluidic and Capillary Electrophoresis ApplicationsElectrowetting and Microfluidic Technologies