Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
Adele Smart, Cristiana Tisca, István N. Huszár, Daniel Kor, Olaf Ansorge, Mohamed Tachrount, Sean Smart, Jason P. Lerch, Karla L. Miller, Aurea B. Martins-Bach
Abstract
Combining histology and ex vivo MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for ex vivo MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse brain dissection, tissue processing, paraffin embedding, sectioning, and staining. We then detail registration of histology to ex vivo MRI data from the same sample and extraction of quantitative histological measurements.
Topics & Concepts
Ex vivoHistologyPathologyIn vivoStainingBiomedical engineeringMedicineBiologyBiotechnologyMolecular Biology Techniques and ApplicationsAdvanced MRI Techniques and ApplicationsRNA Research and Splicing