Deciphering a Cyclodipeptide Synthase Pathway Encoding Prenylated Indole Alkaloids in Streptomyces leeuwenhoekii
Yuqiao Zhang, Tingting Yao, Yuechen Jiang, Huayue Li, Weicheng Yuan, Wenli Li
Abstract
(l-Trp-l-Trp) (cWW)-synthesizing CDPS SazA and the unusual prenyltransferase (PT)-methyltransferase (MT) fused enzyme SazB were characterized. Our results expand the repertoire of CDPSs and associated tailoring enzymes, setting the stage for accessing diverse prenylated alkaloids using synthetic biology strategies.
Topics & Concepts
Drug discoveryPrenylationATP synthaseIndole testBiologyComputational biologyStreptomycesBiochemistryGeneticsGeneEnzymeBacteriaMicrobial Natural Products and BiosynthesisNatural product bioactivities and synthesisPlant biochemistry and biosynthesis