Sex-specific responses to slow progressive pressure overload in a large animal model of HFpEF
Deborah Eaton, Remus M. Berretta, Jacqueline E. Lynch, Joshua G. Travers, Ryan Pfeiffer, Michelle L. Hulke, Huaqing Zhao, Alexander R. H. Hobby, Giana Schena, Jaslyn Johnson, Markus Wallner, Edward Lau, Maggie P. Y. Lam, Kathleen C. Woulfe, Nathan R. Tucker, Timothy A. McKinsey, Marla R. Wolfson, Steven R. Houser
Abstract
We performed a comprehensive assessment to evaluate the effects of slow progressive pressure overload on cardiopulmonary function in a large animal model of heart failure with preserved ejection fraction (HFpEF) in males and females. Functional and structural assessments were performed at the organ, tissue, cellular, protein, and transcriptional levels. This is the first study to compare snRNAseq and ECM mass spectrometry of HFpEF myocardium from males and females. The results broaden our understanding of the pathophysiological response of both sexes to pressure overload. Both sexes developed a robust cardiopulmonary phenotype, but the phenotype was equal or a bit less robust in females.