Bifidobacterium mizhiense sp. nov., isolated from the gut of honeybee (Apis mellifera)

Abstract

A novel bifidobacteria (designated S053-2 T ) was isolated from the gut of honeybee ( Apis mellifera ). Strain S053-2 T was characterized using a polyphasic taxonomic approach. The result of 16S rRNA gene sequence analysis indicated that strain S053-2 T was phylogenetically related to the type strains of Bifidobacterium asteroides , Bifidobacterium indicum , Bifidobacterium actinocoloniiforme , Bifidobacterium xylocopae , Bifidobacterium coryneforme , Bifidobacterium apousia , Bifidobacterium choladohabitans and Bifidobacterium polysaccharolyticum , and had 95.5–99.7 % 16S rRNA gene sequence similarities. Based on the 16S rRNA gene sequence analysis, strain S053-2 T was most closely related to the type strain of B. asteroides , having 99.7 % 16S rRNA gene sequence similarity. Strain S053-2 T had relatively low (91.6–95.7 %) pheS , atpA , clpC , dnaG , fusA , glnA , glyS , hsp60 , argS , pyrG and recA sequence similarities to the type strain of B. asteroides . Strain S053-2 T had 94.5–95.3% atpA , clpC , dnaG , dnaK and pyrG sequence similarities to the type strain of B. apousia . The phylogenomic tree indicated that strain S053-2 T belonged to the B. asteroides group, and was most closely related to the type strains of B. asteroides , B. apousia , B. choladohabitans and B. polysaccharolyticum , and distantly related to type strains of other phylogenetically related species in the B. asteroides group. Strain S053-2 T shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA–DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with B. apousia W8102 T . Strain S053-2 T shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with B. asteroides DSM 20089 T . Acid production from l -arabinose, d -xylose, d -mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l -fucose, and activity of esterase lipase (C8) and α -fucosidase could differentiate strain S053-2 T from B. asteroides DSM 20089 T . Acid production from d -mannose, maltose, sucrose, melezitose and gentiobiose, and activity of α -fucosidase could differentiate strain S053-2 T from <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.41106" xlink:type="simpl

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