Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans
George Q. Huang, Bailey de Jesus, Alex Koh, Sara Blanco, Aubrie Rettmann, Ella DeMott, Melynda Sylvester, Cassie Ren, Carrie Meng, Skye Waterland, Anita Rhodes, Persephone Alicea, Abbey Flynn, Daniel J. Dickinson, Ryan Doonan
Abstract
. 2018; Ghanta and Mello 2020).
Topics & Concepts
CRISPRCas9Gene knockinPlasmidSelection (genetic algorithm)BiologyComputational biologyGeneticsGeneComputer scienceArtificial intelligenceCRISPR and Genetic EngineeringGenetics, Aging, and Longevity in Model OrganismsEvolution and Genetic Dynamics