Nanoscopic dopamine transporter distribution and conformation are inversely regulated by excitatory drive and D2 autoreceptor activity
Matthew D. Lycas, Aske L. Ejdrup, Andreas T. Sørensen, Nicolai O. Haahr, Søren Jørgensen, Daryl A. Guthrie, Jonatan Fullerton Støier, Christian Werner, Amy Hauck Newman, Markus Sauer, Freja Herborg, Ulrik Gether
Abstract
channels. The DAT nanodomains contain tens of transporters molecules and overlap with nanodomains of PIP2 (phosphatidylinositol-4,5-bisphosphate) but show little overlap with D2 autoreceptor, syntaxin-1, and clathrin nanodomains. The data reveal a mechanism for rapid alterations of nanoscopic DAT distribution and show a striking link of this to the conformational state of the transporter.
Topics & Concepts
AutoreceptorExcitatory postsynaptic potentialBiophysicsChemistryDopamine transporterCell biologyTransporterNeuroscienceDopamineDopaminergicBiologyBiochemistryReceptorGeneSerotoninAdvanced Fluorescence Microscopy TechniquesCellular transport and secretionLipid Membrane Structure and Behavior