Protocol for bacterial typing using Fourier transform infrared spectroscopy
Huayan Yang, Haimei Shi, Bin Feng, Li Wang, Liangqiang Chen, Avelino Álvarez‐Ordóñez, Li Zhang, Hao Shen, Jianhua Zhu, Shouning Yang, Chuan‐Fan Ding, Miguel Prietod, Fan Yang, Shaoning Yu
Abstract
The Fourier transform infrared (FT-IR) signals obtained from bacterial samples are specific and reproducible, making FT-IR an efficient tool for bacterial typing at a subspecies level. However, the typing accuracy could be affected by many factors, including sample preparation and spectral acquisition. Here, we present a unified protocol for bacterial typing based on FT-IR spectroscopy. We describe sample preparation from bacterial culture and FT-IR spectrum collection. We then detail FT-IR spectrum preprocessing and multivariate analysis of spectral data for bacterial typing.