An aptamer-exonuclease III (Exo III)–assisted amplification-based lateral flow assay for sensitive detection of Escherichia coli O157:H7 in milk
Yuwei Ren, Pingping Gao, Yang Song, Xinyan Yang, Tao Yang, Sihan Chen, Shiqian Fu, Xue Qin, Meili Shao, Chaoxin Man, Yujun Jiang
Abstract
cfu/mL. This LFA was highly specific to E. coli O157:H7, and the time for detection of E. coli O157:H7 in milk was 4 h. Hence, this system has important application prospects in the detection of pathogenic bacteria in dairy products.
Topics & Concepts
AptamerEscherichia coliDetection limitExonucleaseBacteriaDNAChemistryExonuclease IIIMolecular biologyBiologyBiochemistryChromatographyGenePolymeraseGeneticsAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionBacteriophages and microbial interactions