Identification and characterization of <i>in silico</i>, <i>in vivo</i>, <i>in vitro</i>, and reactive metabolites of infigratinib using LC-ITMS: bioactivation pathway elucidation and <i>in silico</i> toxicity studies of its metabolites
Nasser S. Al‐Shakliah, Mohamed W. Attwa, Adnan A. Kadi, Haitham AlRabiah
Abstract
An <italic>in silico</italic> web designer tool was utilized to guide laboratory work for infigratinib metabolism. Sixteen metabolites of infigratinib and seven reactive intermediates (three iminium ions and four 1,4 benzoquinones) were characterized using LC-ITMS.
Topics & Concepts
In vivoChemistryGlutathioneIn silicoIn vitroGlucuronidationBiochemistryMetabolismMicrosomePharmacologyEnzymeBiologyBiotechnologyGeneColorectal Cancer Treatments and StudiesPancreatic and Hepatic Oncology ResearchPeptidase Inhibition and Analysis