Metabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas
Bruna de Falco, Francesco Giannino, Fabrizio Cartenì, Stefano Mazzoleni, Dong‐Hyun Kim
Abstract
C-MFA, starting from the preparation of cell cultures and labelled tracers to the quenching and extraction of metabolites and their subsequent analysis performed with very powerful software. Here, the limitations and advantages of nuclear magnetic resonance spectroscopy and mass spectrometry techniques used in carbon labelled experiments are elucidated by reviewing the most recent published papers. Furthermore, we summarise the most successful approaches used for computational modelling in flux analysis and the main application areas with a particular focus in metabolic engineering.
Topics & Concepts
Metabolic flux analysisFlux (metallurgy)Pathway analysisSample (material)Biological systemComputer scienceComputational biologyBiochemical engineeringChemistryBiologyChromatographyEngineeringBiochemistryMetabolismGene expressionGeneOrganic chemistryMicrobial Metabolic Engineering and BioproductionMetabolomics and Mass Spectrometry StudiesGene Regulatory Network Analysis