Phosphitispora fastidiosa gen. nov. sp. nov., a new dissimilatory phosphite-oxidizing anaerobic bacterium isolated from anaerobic sewage sludge
Zhuqing Mao, Fabian Gräßle, Jasmin Frey, Paolo Franchini, David Schleheck, Nicolai Müller, Bernhard Schink
Abstract
A new strictly anaerobic bacterium, strain DYL19 T , was enriched and isolated with phosphite as the sole electron donor and CO 2 as a single carbon source and electron acceptor from anaerobic sewage sludge sampled at a sewage treatment plant in Constance, Germany. It is a Gram-positive, spore-forming, slightly curved, rod-shaped bacterium which oxidizes phosphite to phosphate while reducing CO 2 to biomass and small amounts of acetate. Optimal growth is observed at 30 °C, pH 7.2, with a doubling time of 3 days. Beyond phosphite, no further inorganic or organic electron donor can be used, and no other electron acceptor than CO 2 is reduced. Sulphate inhibits growth with phosphite and CO 2 . The G+C content is 45.95 mol%, and dimethylmenaquinone-7 is the only quinone detectable in the cells. On the basis of 16S rRNA gene sequence analysis and other chemotaxonomic properties, strain DYL19 T is described as the type strain of a new genus and species, Phosphitispora fastidiosa gen. nov., sp. nov.