Evaluation of Cytotoxic Activity of Galantamine Peptide Esters: GAL-LEU and GAL-VAL Against PC3 Cell Line
Dobrina Tsvetkova, L. Vezenkov, Tchavdar Ivanov, Dancho Danalev, Ivanka Kostadinova
Abstract
The focus of the present study was to evaluate the cytotoxic effects of novel peptide esters derived from galantamine—GAL-LEU and GAL-VAL—on the PC3 prostate cancer cell line. To assess the cytotoxicity of these compounds, the MTT assay, a method that quantifies cellular metabolic activity through the reduction of MTT to formazan, was used. PC3 cells were exposed to different concentrations (ranging from 1.875 μM to 30 μM) of the peptide esters in triplicate. The absorbance of the formazan produ
Topics & Concepts
GalantamineCytotoxic T cellPeptideChemistryCell cultureBiochemistryBiologyInternal medicineMedicineIn vitroGeneticsDementiaDonepezilDiseaseSynthesis and pharmacology of benzodiazepine derivativesSynthesis and bioactivity of alkaloidsCancer therapeutics and mechanisms