Liposome Delivery of Nucleic Acids in Bacteria: Toward <i>In Vivo</i> Labeling of Human Microbiota
Luis Fernando Moreira, Nuno Guimarães, Sara Pereira, Rita S. Santos, Joana A. Loureiro, Maria do Carmo Pereira, Nuno F. Azevedo
Abstract
. Furthermore, a structural and morphological assessment of the changes on bacteria allowed us to observe that liposomes increased the permeability of the cell envelope especially in Gram-negative bacteria. Considering the delivery efficiency and permeabilization effect, lipid concentrations of approximately 5 mM should be selected to maximize the detection of bacteria without compromising the bacterial cellular structure.
Topics & Concepts
BacteriaNucleic acidLiposomeBiologyIn vivoGram-negative bacteriaLysisGram-positive bacteriaBiochemistryMicrobiologyBacterial cell structureEscherichia coliBiotechnologyGeneticsGeneBacteriophages and microbial interactionsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery