Macromolecular Photocatalyst for Synthesis and Purification of Protein–Polymer Conjugates
Rebecca Olson, Jordan S. Levi, Georg M. Scheutz, Jacob J. Lessard, C. Adrian Figg, Manasi Kamat, Kari B. Basso, Brent S. Sumerlin
Abstract
We describe the application of a novel eosin Y (EY)-derived polymer photocatalyst for the synthesis of polymeric bioconjugates. The photocatalyst, a copolymer of eosin Y acrylate and N-isopropylacrylamide, can induce light-mediated reversible-deactivation radical polymerization in biologically benign conditions. Heating the reaction mixture to 37 °C causes precipitation of the photocatalyst in a hydrophilic-to-hydrophobic transition, allowing for simple purification of the polymer–protein conjugates via filtration without compromising enzyme activity. We discuss the optimization of the polymerization conditions for imparting control over molecular weight and reaction kinetics and demonstrate the recyclability of the recovered photocatalyst. Overall, this strategy will advance bioconjugate manufacturing through facilitated purification and improved sustainability.