Human Hexa-Histidine-Tagged Single-Chain Variable Fragments for Bioimaging of Bacterial Infections
Thae Thae Min, Montarop Yamabhai
Abstract
were used as model Gram-positive and Gram-negative bacteria, respectively. Specificity at the strain and species level to both planktonic and biofilm forms of these bacteria were demonstrated in various assay formats, namely, ELISA, flow cytometry, western blot, immunofluorescence, and electron microscopy via the hexa-histidine tag. This recombinant scFv generation platform can be applied for other bacteria, and since the scFv obtained has a benefit of being a human origin, it could be conveniently engineered for various therapeutic or theranostic applications with minimized adverse immunoreaction.
Topics & Concepts
Recombinant DNASingle-chain variable fragmentEscherichia coliAntibodyBacteriaPhage displayMicrobiologyMonoclonal antibodyBacterial cell structureChemistryBiologyBiochemistryImmunologyGeneGeneticsMonoclonal and Polyclonal Antibodies ResearchBacteriophages and microbial interactionsAdvanced Biosensing Techniques and Applications