Fc-specific and covalent conjugation of a fluorescent protein to a native antibody through a photoconjugation strategy for fabrication of a novel photostable fluorescent antibody
Xiaotian Yu, Xiao-Yan Fu, Xiaoyi Gao, Xiao-kun Zhang, Shujuan Liang, Hong‐Ming Yang, Jinbao Tang
Topics & Concepts
PhotobleachingChemistryFluorescenceImmunofluorescenceFluorophoreGreen fluorescent proteinCovalent bondAntibodyImmunoassayFörster resonance energy transferProtein engineeringLinkerMolecular biologyBiophysicsBiochemistryEnzymeBiologyComputer scienceGenePhysicsQuantum mechanicsImmunologyOrganic chemistryOperating systemMonoclonal and Polyclonal Antibodies ResearchRNA and protein synthesis mechanismsClick Chemistry and Applications