<i>FKS1</i> Is Required for Cryptococcus neoformans Fitness <i>In Vivo</i> : Application of Copper-Regulated Gene Expression to Mouse Models of Cryptococcosis
Sarah R. Beattie, Andrew J. Jezewski, Laura C. Ristow, Melanie Wellington, Damian J. Krysan
Abstract
Conditional expression systems are widely used to genetically validate antifungal drug targets in mouse models of infection. Copper-regulated expression using the promoter of the CTR4 gene has been sporadically used for this purpose in C. neoformans .
Topics & Concepts
Cryptococcus neoformansCryptococcosisGene expressionBiologyIn vivoGeneMicrobiologyAntifungalAntifungal drugGeneticsFungal Infections and StudiesAntifungal resistance and susceptibilityNail Diseases and Treatments