The Immunocytochemical Identification of PIWI-Positive Cells during the Recovery of a Coelomocyte Population after Evisceration in the Holothurian Eupentacta fraudatrix (Djakonov et Baranova, 1958) (Holothuroidea: Dendrochirota)
Eugenia G. Zavalnaya, Ekaterina V. Shamshurina, М. Г. Елисейкина
Abstract
Abstract The quantitative dynamics of coelomic fluid cells of Eupentacta fraudatrix (Djakonov et Baranova, 1958) were studied by flow cytometry within a day after evisceration. A differently directed dynamics pattern was noted among groups of poorly differentiated juvenile cells and differentiated cells during regeneration. The juvenile coelomocyte fraction increased, while the content of differentiated cells decreased and vice versa. The use of PIWI protein as a pluripotent stem cell marker allowed us to distinguish a PIWI-positive cell subset among juvenile coelomocytes. Their content in the coelomic fluid was highest during early regeneration at 1 and 4 h after evisceration and decreased at 24 h after evisceration.