Inertial focusing of circulating tumor cells in whole blood at high flow rates using the microfluidic CTCKey™ device for CTC enrichment
Kaylee Smith, Jessica Antoinette Jana, Anna Kaehr, Emma Purcell, Tyler Opdycke, Costanza Paoletti, Laura Cooling, Douglas H. Thamm, Daniel F. Hayes, Sunitha Nagrath
Abstract
, the CTCKey™ reduces the CTC containing blood volume by 78%; the CTCs from 1 mL of blood are now in 0.22 mL of blood. The CTCKey's™ ability to concentrate CTCs from a large original blood volume to a smaller, highly concentrated volume enables a much greater blood volume to be interrogated by downstream isolation and characterization methods despite their low volume input limitations.
Topics & Concepts
Circulating tumor cellWhole bloodBlood volumeBlood flowMicrofluidicsBiomedical engineeringFlow cytometryCancerMedicineInternal medicineMaterials scienceImmunologyNanotechnologyMetastasisMicrofluidic and Bio-sensing TechnologiesCancer Cells and Metastasis3D Printing in Biomedical Research